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. 2017 Feb 28;17:56. doi: 10.1186/s12870-017-1003-9

Table 3.

Two-way ANOVA and multiple linear regression analysis (MLR) of the relative gene expression in Brassica juncea seedlings raised from EBR-soaked seeds and grown in IMI supplemented substratum

Gene name F-ratios HSD (p < 0.05)
FIMI FEBR FIMI×EBR
SOD 0.78 48.57*** 0.01 0.26
CAT 16.66** 87.07*** 6.89* 0.45
GR 7.05* 124.34*** 42.83*** 0.41
RBOH 19.06** 0.46 31.10*** 0.66
NADH 4.16 51.72*** 18.29** 0.46
CXE 21.64** 88.35*** 2.26 0.56
GSH-S 0.93 10.57* 2.86 0.46
GSH-T 0.59 17.10** 1.14 0.60
GST-1 21.14** 26.40*** 8.68* 0.40
GST-2 6.86* 143.74*** 0.52 0.49
GST-3 12.82** 99.63*** 1.85 0.90
GST-4 0.09 8.38* 1.07 0.43
GST-5 0.19 110.10*** 0.11 1.06
GST-6 0.059 143.70*** 5.28 1.21
POD 40.71*** 23.44** 1.66 1.24
P450 11.03* 51.62*** 4.06 0.96
MLR equation β-regression coefficient Multiple correlation coefficient Significant at p <
βIMI βEBR
SOD expression = 0.9960 + 0.0003 X1 + 0.0041 X 2 0.1169 0.9201 0.9274 0.001
CAT expression = 1.1311–0.0020 X1 + 0.0093 X 2 – 0.3748 0.8566 0.9351 0.001
GR expression = 1.3034 + 0.0012 X1 + 0.0103 X 2 0.1968 0.8260 0.8491 0.001
RBOH expression = 1.4136 + 0.0032 X1–0.0010 X 2 0.5702 – 0.0892 0.5771 0.10
NADH expression = 1.2212 + 0.0011 X1 + 0.0074 X 2 0.2250 0.7933 0.8246 0.01
CXE expression = 0.9051 + 0.0029 X1 + 0.0118 X 2 0.4242 0.8571 0.9563 0.001
GSH-S expression = 0.9113 + 0.0005 X1 + 0.0034 X 2 0.2040 0.6875 0.7170 0.02
GSH-T expression = 1.0713 + 0.0005 X1 + 0.0055 X 2 0.1483 0.7983 0.8120 0.01
GST-1 expression = 0.8681 + 0.0021 X1 + 0.0046 X 2 0.5737 0.6411 0.8604 0.001
GST-2 expression = 1.0400 + 0.0015 X1 + 0.0133 X 2 0.2077 0.9504 0.9728 0.001
GST-3 expression = 0.8635 + 0.0036 X1 + 0.0200 X 2 0.3238 0.9025 0.9588 0.001
GST-4 expression = 0.9497 + 0.0002 X1–0.0030 X 2 0.0747 – 0.6909 0.6949 0.02
GST-5 expression = 1.0393 + 0.0005 X1 + 0.0248 X 2 0.0401 0.9643 0.9651 0.001
GST-6 expression = 1.3087 + 0.0003 X1 + 0.0322 X 2 0.0195 0.9565 0.9567 0.001
POD expression = 1.1777 + 0.0088 X1 + 0.0134 X 2 0.7426 0.5635 0.9322 0.001
P450 expression = 1.2155–0.0036 X1 + 0.0154 X 2 – 0.3842 0.8312 0.9157 0.001

X1 = IMI (mg L−1), X 2 = EBR (nM), *, ** and *** = significant at p < 0.05, p < 0.01 and p < 0.001 respectively