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. 2016 Jan 12;17:4. doi: 10.1186/s12931-016-0320-0

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© Dong et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of BMAL1 on TGF-β1-induced FMD. NHLFs were transiently transfected with siBMAL1 or control siRNA (siCtrl) for 48 h. During the last 24 h of transfection, the cells were treated with TGF-β1 at 1 ng/ml. Real-time qRT-PCR analysis was employed to investigate the expression of α-SMA (a) and PAI1 (b), * p < 0.05 vs. siCtrl-NT; ** p < 0.05 vs. siCtrl-TGF-β1. c Western blot analysis was carried out to investigate the TGF-β1-induced α-SMA, PAI1 and col-1 expression in response to BMAL1 knockdown. Densitometry analysis by ImageJ is presented. * p < 0.05 vs. corresponding control siRNA groups stimulated with TGF-β1 (siCtrl-TGF-β1)