Table 1.
Primer sequences for RT-PCR
| Gene | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') |
|---|---|---|
| h-α-SMA | GAAGAAGAGGACAGCACT | TCCCATTCCCACCATCAC |
| h-Collagen-1 | CGGAGGAGAGTCAGGAAGG | CACAAGGAACAGAACAGAACA |
| 36B4 | CGACCTGGAAGTCCAACTAC | ATCTGCTGCATCTGCTTG |
| h-E-cad | ATTCTGATTCTGCTGCTCTTG | AGTCCTGGTCCTCTTCTCC |
| h-PAI1 | GGCTGGTGCTGGTGAATGC | AGTGCTGCCGTCTGATTTGTG |
| h-FN | GGAGAGAGTCAGCCTCTGGTTCAG | AGTCCACTGGGCGCTCAGGCTTGTG |
| m-BMAL1 | TTTGGGCTAGCTGTGGATAG | AAATATCCACATGGGGGACT |
| m-Per1 | CATCTCAGCGGAGTTCTCAT | CTGGTAGATGGGTTGTCCTG |
| m-Per2 | CTGACGCACACAAAGAACTG | GTAGGAAGGCACATCCACAC |
| m-Per3 | GAGGCACACTAAGCCCAGTA | CGCGAAGGTATCTGTGTTCT |
| m-Npas2 | TGGGAATCCACGATAAGAAA | GGAATTCTGGAATCGTTGTG |
| m-Nr1d1 | GCTTCTCTCAGTTCCCACAA | GGCATTGAAGTTACCAGGTG |
| m-Nr1d2 | TGCAGAATGACACCTTAGCA | GGCATGCTCTCAGATGAGTT |
| m-DBP | ACCTGACTTCCTCCTTGTCC | CCCGGGTTCTCAAGATTTAT |
| h-BMAL1 | GGAAAAATAGGCCGAATGAT | TGAGCCTGGCCTGATAGTAG |
| h-MMP9 | TGACAGCGACAAGAAGTG | CAGTGAAGCGGTACATAGG |