Figure 3. Effect of MIP on DC survival. BMDCs were stimulated with MIP(K) and MIP(L) at an MOI of 10 and analyzed for Annexin V and PI staining after 24 h. Lower levels of Annexin V and PI-positive cells were observed in MIP(K)- and MIP(L)-stimulated DCs (A). Both MIP(K) and MIP(L) enhanced DC survival in a dose-dependent manner when used at MOIs of 5, 10, and 20 (B). Mean ± sem of 3 independent experiments is shown. **P < 0.01; ns, not significant. Expression of antiapoptotic proteins Bcl-2 and Bcl-xl was analyzed in unstimulated and MIP-stimulated BMDCs by Western blotting. MIP(K) induced the expression of Bcl-xl, whereas MIP(L) enhanced the expression of both Bcl-2 and Bcl-xl (C). Fold expression to unstimulated DCs is indicated with respective bands. Representative data of 2 independent experiments are shown.