Figure 6. Phenotypic characterization of freshly isolated Ficoll-isolated kidney lymphocytes. (A) Representative flow cytometric density plots show the proportion of NK cells in the 4 mouse groups. (B) Cumulative data representative of the mean percentage and total number of CD45⁺NKp46⁺CD3⁻ cells in all 4 groups. (C) NK cells were gated as CD45⁺NKp46⁺CD3⁻ and stained for various NK cell markers. Gates were set by use of unstained or nonspecific mAb isotype controls; a representative staining of each marker is shown. The median fluorescence intensity for each marker was analyzed. (D) NK cells were analyzed for CD11bCD27 subsets, and the percentages of CD11b^HighCD27^Low, CD11b^HighCD27^High, and CD11b^LowCD27^High cells were calculated. Gates for each population were set by use of unstained or nonspecific mAb isotype controls. Data were analyzed with a 2-tailed, unpaired Student’s t-test (mean ± sd; n = 4–6 mice/group in 3–4 independent experiments; **P < 0.01; *P < 0.05).