Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1968 Dec;16(12):1913–1918. doi: 10.1128/am.16.12.1913-1918.1968

Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium

P G Caltrider 1, F M Huber 1, L E Day 1
PMCID: PMC547795  PMID: 16349829

Abstract

The metabolism of Cephalosporium acremonium grown in a complex medium supplemented with DL-methionine or inorganic sulfate was studied. More growth occurred in a sulfate medium than in a methionine medium. Methionine-grown cells had an increased rate of respiration, a higher rate of catabolism with acetate and glucose as substrate, and higher specific activities of certain respiratory enzymes than sulfate-grown cells. Labeled acetate and glucose were assimilated at a faster rate by methionine-grown cells than sulfate-grown cells. Taurine, cystathionine, and small quantities of four acidic compounds were present in the amino acid pool of methionine-grown cells, but they were not detected in the pool of sulfate-grown cells. The differences in metabolic activity of sulfate and methionine-grown cells are discussed in regard to cephalosporin C synthesis.

Full text

PDF
1918

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bent K. J., Morton A. G. Amino acid composition of fungi during development in submerged culture. Biochem J. 1964 Aug;92(2):260–269. doi: 10.1042/bj0920260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARK E., BOWBURY R. J. STUDIES OF METHIONINE SYNTHESIS IN COPRINUS LAGOPUS. J Gen Microbiol. 1964 Sep;36:333–339. doi: 10.1099/00221287-36-3-333. [DOI] [PubMed] [Google Scholar]
  3. Caltrider P. G., Niss H. F. Role of methionine in cephalosporin synthesis. Appl Microbiol. 1966 Sep;14(5):746–753. doi: 10.1128/am.14.5.746-753.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DELAVIER-KLUTCHKO C., FLAVIN M. ENZYMATIC SYNTHESIS AND CLEAVAGE OF CYSTATHIONINE IN FUNGI AND BACTERIA. J Biol Chem. 1965 Jun;240:2537–2549. [PubMed] [Google Scholar]
  5. DEMAIN A. L., NEWKIRK J. F. Biosynthesis of cephalosporin C. Appl Microbiol. 1962 Jul;10:321–325. doi: 10.1128/am.10.4.321-325.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FISCHER G. A. The cleavage and synthesis of cystathionine in wild type and mutant strains of Neurospora crassa. Biochim Biophys Acta. 1957 Jul;25(1):50–55. doi: 10.1016/0006-3002(57)90415-8. [DOI] [PubMed] [Google Scholar]
  7. Keay L., Crook E. M. Effect of metal ions on hog liver esterase. Arch Biochem Biophys. 1965 Sep;111(3):626–634. doi: 10.1016/0003-9861(65)90244-4. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  10. Ott J. L., Godzeski C. W., Pavey D., Farran J. D., Horton D. R. Biosynthesis of Cephalosporin C: I. Factors Affecting the Fermentation. Appl Microbiol. 1962 Nov;10(6):515–523. doi: 10.1128/am.10.6.515-523.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  12. Smith B., Warren S. C., Newton G. G., Abraham E. P. Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp. Biochem J. 1967 Jun;103(3):877–890. doi: 10.1042/bj1030877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TROWN P. W., SMITH B., ABRAHAM E. P. Biosynthesis of cephalosporin C from amino acids. Biochem J. 1963 Feb;86:284–291. doi: 10.1042/bj0860284. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES