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. 2017 Jun 20;12(6):e0177432. doi: 10.1371/journal.pone.0177432

Fig 3. PMK-1 affects mtl-1 transcription and translocates to the nucleus in response to cadmium.

Fig 3

(A) pmk-1 gene x cadmium interaction factor determined by comparing changes of mtl-1 mRNA levels in pmk-1 mutants to either wild type N2 or atf-7(gk715) mutant after exposure to 100 μM cadmium for 1 (dark gray) h or 5 (light gray) h. (B) C. elegans strain pmk-1::GFP, which expresses a PMK-1::GFP fusion protein was exposed to 0, 25, 100 or 200 μM cadmium for 5 h. (C) Percentage of pmk-1::GFP animals in which PMK-1::GFP was localized in intestinal nuclei in response to indicated concentrations of cadmium and (D) after exposure to both cadmium and akt-1(RNAi);akt-2(RNAi) (light gray) or vector RNAi (white). Means ± S.E.M. for four biological replicates are presented. * p < 0.05 and ** p < 0.01.