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. 2017 Jun 20;12(6):e0179099. doi: 10.1371/journal.pone.0179099

Fig 5. MCD diet induces NASH independently of IL-6 signalling.

Fig 5

Liver samples from 4 weeks-fed control diet (CD) or methionine and choline deficient diet (MCDD) mice were homogenised and (A) mRNA expression of pro-inflammatory genes and macrophage marker was assessed by real time PCR and (B) protein expression of IL-6 and the loading control αTubulin was measured by Western blotting in the WT or transgenic animals. (C) Plasma and hepatic IL6 levels were measured in C57BL/6 fed a CD or MCDD for 4 weeks and injected with vehicle or sgp130Fc every second day. Data expressed as average ± SEM, n = 6–7. Statistical analysis by 2 way ANOVA, * p<0.05; **p<0.01; ***p<0.001 Main effect for Control vs MCDD; & p<0.05 for diet/genotype interaction.