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. Author manuscript; available in PMC: 2017 Jul 16.
Published in final edited form as: ACS Chem Biol. 2016 Nov 15;11(12):3305–3309. doi: 10.1021/acschembio.6b00940

Table 1. Catalytic parameters of wild-type alanine racemase and its mutants.

Enzyme kcat (s-1) Rel. kcat KM (mM) kcat/KM (M-1s-1) Rel. kcat/KM
wild type 3.5 (0.1) × 103 1 2.2 1.6 (0.2) × 106 1
H166Ta 1.7 (0.1) × 101 0.005 0.5 3.3 (0.2) × 104 0.02
H166Mh 8.4 (0.3) × 100 0.002 0.5 1.8 (0.2) × 104 0.01
H200Ta 1.2 (0.5) × 101 0.003 1.6 7.3(0.8) × 103 0.005
H200Mh 5.2 (0.2) × 100 0.002 1.6 3.2 (0.4) × 103 0.002
H127Ta 1.2 (0.4) × 101 0.003 1.2 1.0 (0.1) × 104 0.006
H127Mh 2.2 (0.1) × 101 0.006 1.8 1.2 (0.2) × 104 0.007
H125Ta 4.1 (0.2) × 103 0.8 4.7 8.7 (0.4) × 105 0.5
H125A 4.4 (0.2) × 102 0.08 0.5 8.2 (0.4) × 105 0.5
H166A 3.5 (0.2) × 100 0.001 1.6 2.2 (0.3) × 103 0.001
H166F 3.9 (0.1) × 100 0.001 3.9 1.0 (0.1) × 103 0.0006
H127A/H166A/H200A 2.8 (0.4) × 10-1 0.00008 0.8 3.6(0.5) × 102 0.0002