Fig. 1.

Selection and screening of positive phage by cell ELISA on CLDN-1-positive and -negative cells. (a) Schematic representation of parallel and crossed selection on different cell lines. Positive phage, which selectively bound to each CLDN-1-positive cell line, were submitted to two additional rounds of selection, using either the same combination of cells or crossing the selection with the other one. (b) Binding to HEK 293-CLDN-1 of phage selected either by conventional panning on Huh7.5 or HEK 293-CLDN-1 cells in suspension (black bars), or by crossed selections (grey bars) on both Huh7.5 and HEK 293-CLDN-1 cells and indicated with (X), or by panning on adherent CHO cells (hatched bars) and indicated with (C). As a control, all the selected phage were tested on control mock-transduced HEK 293 cells (white bars). Error bars indicate sd.