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. 2017 Jun 21;37(7):1361–1370. doi: 10.1161/ATVBAHA.116.308929

Figure 3.

Figure 3.

Effects of serotonin on oxidation of protein tyrosine phosphatases (PTPs) and global protein carbonylation. Irreversible oxidation of PTPs using the oxPTP antibody, in response to serotonin in human pulmonary artery smooth muscle cells (hPASMCs) in the presence or absence of SB224289 or Nox1 inhibitor, ML171 (A). PTP oxidation in wild-type (WT) and Nox1−/− mouse pulmonary artery smooth muscle cells (mPASMCs) treated with serotonin (B). Total protein carbonylation in response to serotonin in hPASMCs (2 h; C) and in Nox1−/− mPASMCs (1 h; D). Results are representative of 3 to 5 experiments where protein expression is relative to β-actin. *P<0.05, **P<0.01, vs vehicle control hPASMCs or WT vehicle mPASMCs; †P<0.05 vs treated WT mPASMCs; ‡‡P<0.01 vs vehicle pulmonary arterial hypertension (PAH)-hPASMCs; §P<0.05, §§P<0.01 vs serotonin-treated human pulmonary artery smooth muscle cells from PAH subjects (PAH-hPASMCs) determined by ANOVA with Tukey post hoc test. ML indicates ML171; SB, SB224289; Ser, serotonin; and V, vehicle.