Fig. 4.
KRAS-induced transformation of epithelial MCF-10A can be partially prevented by knockdown of RIOK1. (a) RIOK1 knockdown Western blot analysis for MCF-10A KRAS cells (N = 1). (b and c) Colony formation assays of MCF-10A KRAS cells. Formed colonies were stained after 14 days and cell density was determined. Representative pictures are shown. Data is represented as mean ± SEM with **** for p < 0.0001, *** for p < 0.001, ** for p < 0.01 and * for p < 0.05 by ordinary two-way ANOVA (Tukey); N = 3. (d) MTT assay measured on indicated timepoints for MCF-10A KRAS cells. Data is represented as mean ± SEM with *** for p < 0.001, ** for p < 0.01 and * for p < 0.05 by two-way ANOVA (Tukey); N = 3. (e) MCF-10A KRAS cells were seeded in 2% Matrigel and grown for 28 days. Scale bars: 200 μm. Black arrows indicate invasive protrusion formed in the GFP/KRASG12V transformed cells. Note that these are lost upon RIOK1 knockdown (white arrows) (See also Fig. S3).