Fig. 5.

SILAC-based mass-spectrometric approach and validation of RIOK1 effector proteins. (a) NCI-H1299 shRIOK1 cells were labeled by SILAC and induced or left untreated 6 days prior to lysis. Lysates were mixed and analyzed by LC-MS/MS. (b) SILAC-based fold-changes of up- and down-regulated proteins upon RIOK1 knockdown are shown. Red rhombuses indicate significantly changed proteins upon knockdown (significance B, permutation based FDR < 0.05). (c) Protein-protein interaction network for significantly regulated proteins upon Riok1 depletion. (d) Representative Western blot analysis and quantifications of NCI-H1299 cells for cell cycle proteins and (e) RIOK1 effector proteins N = 4; *** for p < 0.001; ** for p < 0.01 and * for p < 0.05; unpaired two-tailed t-test (mean ± SEM). (See also Figs. S4 and S5).