Fig. 7.

RIOK1 enables lung colonization. (a) Luciferase expressing NCI-H1299 non-silencing and shRIOK1 cells were injected i.v. in mice and tumor growth was followed using BLI for up to 98 days. (b) Ex vivo fluorescence imaging of isolated lungs on day 100. N = 2. Doxycycline (2 mg/ml) was administered constantly via the drinking water. (c) Representative histological (H&E) and immunohistochemical (RIOK1) staining of the isolated lungs from this experiment and from s.c. injections (Figs. S8 and S9). Arrows point out RIOK1 positive tumor cells. Scale bar upper panel: 200 μm. Scale bar middle and lower panel: 20 μm. (d) Upon s.c. injection (left), NCI-H1299 NS cells preferentially formed nodules of tumor cells (score 2), whereas shRIOK1 cells formed smaller colonies consisting of single cells (score 1). In the case of i.v. injection (right), induced NCI-H1299 shRIOK1 failed to form colonies (score 0), whereas NCI-H1299 NS and non-induced shRIOK1 cells massively infiltrated the lungs of recipient mice (score 3). N = 7 for NS and N = 8 for shRIOK1 for s.c. injections. N = 15 for NS, N = 8 shRIOK1 no dox and N = 16 for shRIOK1 dox for i.v. injection; Chi-square test. (See also Figs. S8 and S9).