Fig. 2.

Effect of long-term hypercholesterolemia on behavioral rhythms under light-dark and group-housing conditions.

(A to F) Long-term monitoring of locomotor activity was performed over a period of up to about 160 days in light and dark. Representative raw activity data of wild-type mice, Ldlr −/− mice (+ HFD) and Ldlr −/− Per2 m/m mice (+ HFD) are shown in (A), (C) and (E), respectively. Average 6-min bin activity was calculated for five-day periods at intervals of about 40 days (days 2–6, 40–44, 80–84 and 120–124), and shown as a line graph. The average ± SE (n = 4 or 8 groups) are shown in (B), (D) and (F), respectively. Yellow shadows indicate light periods. To facilitate visual comparison before and after HFD treatment, gray silhouettes represent data at days 2–6 (normal chow diet).