Fig. 2.

Virus detection by qRT-PCR in tissues and plasma after primary DENV-1 and DENV-2 infection. C57BL/6 mice were infected with (a, b, c, d) DENV-1 or (e, f, g, h) DENV-2 i.p., and at different times p.i. (0, 6, 18, 24, 48, 72, 96, and 168 h), mice were euthanized and (b, f) draining lymph nodes (LN) (mesenteric, periportal and celiac), (c, g) organs (spleen, liver and kidney) and (d, h) plasma were collected. Total RNA was extracted from tissues and DENV RNA was detected by qRT-PCR. Arrows indicate transient viral replication. LN: lymph node. Values represent means ± SEM (n = 6–8/group). Two independent experiments were performed that showed similar results. *p < 0.05, **p < 0.01, ***p < 0.001 determined by one-way ANOVA (dashed line) and Bonferroni's multiple comparison post-test (solid line). The statistical significance of post-test comparisons is only depicted for the DENV RNA peaks that suggest transient replication. Dotted lines show limits of detection.