Fig. 2.

CD147 deletion in T cells lead to an increase of PLZF⁺ NKT-like cells. A. Analysis of PLZF protein expression in the thymus of WT and CD147^T-KO mice by western blot. B—C. Analysis of PLZF⁺ cells in the thymus (B) and in liver (C) of WT and CD147^T-KO mice using flow cytometry. D. Bone marrow hematopoietic stem cells were co-cultured on OP9-DL1 cells without IL-2, and then collected after 7 days. The CD4 vs. CD8, TCRβ and CD25 vs. CD44 populations were detected by flow cytometry. E. Bone marrow hematopoietic stem cells were enriched and co-cultured on OP9-DL1 cells without IL-2, and then collected after 14 days. PLZF⁺ cells were analyzed using flow cytometry. F. DP thymocytes were co-cultured on OP9-DL1 cells without IL-2, and then collected after 21 days. PLZF⁺ cells were analyzed by flow cytometry. G. Analysis of TCRβ expression in PLZF⁺ cells after DP thymocytes were co-cultured on OP9-DL1 cells for 21 days without IL-2. Data are representative 4 (A–C) or 3 (D–G) experiments.