Figure 1. Visceral hypersensitivity in GF mice.
Visceral sensitivity to colorectal distention (CRD; ascending paradigm from 10 to 80 mm Hg) was assessed as the number of visceromotor responses (VMR) over pressures. (A) GF mice displayed increased visceral pain responses compared to controls. (B) The pain threshold was lower in GF compared to CC mice. In this and subsequent figures, *p≤0.05, **p<0.01, and ***p<0.001 versus CC animals. CC, n = 10; GF, n = 9.
Figure 1—figure supplement 1. Schematic illustration showing the ascending phasic distension paradigm (10–80 mmHg) (A) and representative CRD traces at the pressure of 40 mmHg and 65 mmHg for conventional (B,C) or germ-free (D, E) mice. The paradigm consists of three repeated pulses at each pressure level, with a pulse duration of 20 s at 5-min intervals.
