Fig. 2.

The HCS1 antiserum marks mature hair cells in neuromasts. (A) In a triply labeled wild-type larva, α-tubulin is present throughout the neuromast, whereas parvalbumin 3 and HCS1 occur in mature hair cells. (B) In a wild-type larva at 48 hpf, a neuromast deposited by the first primordium already bears mature hair cells labeled for HCS1 (red). The migrating second primordium is negative for this marker. Both structures are labeled by immunoreactivity against claudin b (green). (Scale bars, 10 μm.)