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. 2005 Jan 19;102(5):1731–1736. doi: 10.1073/pnas.0409376102

Fig. 3.

Fig. 3.

The SH3 domains of nArgBP2 bind to actin regulatory proteins. (A) GST or GST-nArgBP2 SH3 domains were used as baits in affinity-purification experiments from a rat brain Triton X-100 extract (BTE, brain Triton X-100 extract). Material retained by the GST fusion proteins, as well as baits only, were resolved by SDS/PAGE and stained with Coomassie blue (Upper). (Lower) Higher-magnification view of the protein bands retained by SH3-2, and methods used for their identification: MALDI-MS, Western blotting (WB), and overlay essay (OE). (B) Western blot analysis of proteins retained by GST-nArgBP2 SH3 domains. SM, starting material; B, bound material; NB, unbound material. (C) GST or GST-nArgBP2 SH3 domains were used as baits in affinity purification experiments from in vitro-translated binding partners. GST was used as negative control. GST fusion proteins used in the pull down essay were resolved by SDS/PAGE and stained with Coomassie blue (Lower).