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. 2005 Jan 19;102(5):1731–1736. doi: 10.1073/pnas.0409376102

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Copyright © 2005, The National Academy of Sciences

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Fig. 4. — Tyrosine phosphorylation and ubiquitination of synaptojanin 2 and WAVE2. (A) Synaptojanin 2b and WAVE2 are phosphorylated by Abl. HEK 293T cells were transfected with plasmids encoding Flag-tagged synaptojanin 1, synaptojanin 2b, WAVE2, and Abl as indicated. Anti-Flag immunoprecipitates were probed with anti-phosphotyrosine antibody. (B) Tyrosine phosphorylation of synaptojanin 2b and WAVE2 is inhibited by the Abl/Arg kinase inhibitor STI571. HEK293T cells were transfected with plasmids encoding Flag-tagged WAVE2 or synaptojanin 2b and Abl in the presence or in the absence of 10 μM Abl kinase inhibitor STI571. Anti-Flag immunoprecipitates were probed with anti-phosphotyrosine antibody. (C) Ubiquitination of synaptojanin 2b and WAVE2. HEK 293T cells were transfected with Flag-tagged synaptojanin 2b or WAVE-2 plus Abl, Cbl, HA-ubiquitin, and either full-length nArgBP2 or nArgBP2 lacking the SH3 domain region (nArgBP2-ΔSH3) as indicated. Anti-Flag immunoprecipitates were probed by Western blotting with anti-HA antibodies (ubiquitin).