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. 2005 Jan 24;102(5):1661–1666. doi: 10.1073/pnas.0407183102

Fig. 3.

Fig. 3.

Effects of eNOS gene transfer in WT and eNOS–/– (NOS3–/–) mice. (a) Representative intracavernous pressure tracings after cavernous nerve stimulation (CNS) at the 6-V setting in WT (Left) and 2-V setting in eNOS–/– (Right) mice. (Inset) The bar graph depicts the frequency of spontaneous erections before and after CNS following AdCMVβgal (Adβgal) or AdCMVeNOS (AdNOS3) transfection. Asterisk indicates P < 0.05 compared to pre-CNS in eNOS–/– mice. (b) Area under the erection response curve (AUC) after direct intracavernosal injection of DEA/NO (0.3 μg/kg) or sildenafil citrate (3 μg/kg) in WT and eNOS–/– (NOS3–/–) mice transfected with Adβgal or AdNOS3. Asterisk indicates P < 0.05 when compared to WT; double asterisk indicates P < 0.05 when compared to NOS3–/– transfected with Adβgal. (c) AUC after termination of CNS (AUC PostCNS) in the presence and absence of sildenafil in WT and NOS3–/– mice transfected with Adβgal or AdNOS3. Asterisk indicates P < 0.05 when compared to WT. (d) Western blot analysis and quantitative densitometry of the protein expression of the phosphorylated (activated) form of PDE5A (p-PDE5A) (Left) and PDE5 enzyme activity at baseline (Right) in WT and NOS3–/– mice transfected with Adβgal or AdNOS3. Asterisk indicates P < 0.05 when compared to WT; double asterisk indicates P < 0.05 when compared to NOS3–/–. For all panels, data are expressed as mean values ± SEM.