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. Author manuscript; available in PMC: 2018 May 10.
Published in final edited form as: Cell Host Microbe. 2017 May 10;21(5):611–618.e5. doi: 10.1016/j.chom.2017.04.001

Figure 1. NADPH oxidase mediates ROS production by neutrophils and facilitates clearance of P. aeruginosa during bacterial keratitis.

Figure 1

(A) Representative images of corneal opacification 24 h post-infection of C57BL/6 and gp91phox −/− (CGD) mice infected with 1x105 CFU PAO1 (WT) or with the ΔpscD (T3SS null) mutant strain. (B) Quantification of corneal opacity by determining average pixel intensity of corneas described previously (Sun et al., 2012)(n = 5 mice). (C) Colony forming units (CFU) recovered from infected corneas 24 h post-infection (n = 9 mice). (D) Corneal sections were stained with hematoxylin and eosin, or (E) 4’,6-Diamidino-2-Phenylindole dye (DAPI, Blue) and an antibody to Ly6G (NIMP-R14, FITC, green) (Epi: epithelium, Str: stroma, End: corneal endothelium, AC: anterior chamber). B, C: Data points represent individual corneas. Median and interquartile range are indicated. Significance was calculated using the Kruskal-Wallis test, with Dunn’s multiple comparison correction. * p<0.05, **p<0.01, ***p<0.001, or n.s., not significant. See also Figure S1.