| Antibodies |
| anti-Akt rabbit polyclonal |
Cell Signaling Technology |
9272 |
| Phospho-Akt rabbit polyclonal |
Cell Signaling Technology |
9275 |
| p40phox
|
Santa Cruz Biotechnology |
Sc-48388 |
| Phospho-p40phox rabbit polyclonal |
Cell Signaling Technology |
4311 |
| Ras rabbit polyclonal |
Cell Signaling Technology |
3965 |
| GRB2 rabbit polyclonal |
Cell Signaling Technology |
3972 |
|
|
|
| Streptavidin APC |
eBioscience |
17-4317-82 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 |
Life Technologies |
A-11006 |
|
|
|
| Bacterial Strains |
| PAO1F (wild-type PAO1) |
(Bleves et al., 2005) |
RP1831 |
| PAO1F ΔpscD
|
(Sun et al., 2012) |
RP1903 |
| PAO1F ΔexoS
|
(Sun et al., 2012) |
RP1883 |
| PAO1F ΔexoT
|
(Sun et al., 2012) |
RP1945 |
| PAO1F ΔexoST
|
(Sun et al., 2012) |
RP1947 |
| PAO1F ΔexoS ΔexoT ΔexoY (Δ3TOX) |
(Cisz et al., 2008) |
RP1949 |
| PAO1F ΔpopBD
|
(Sun et al., 2012) |
RP2750 |
| PAO1F exoS(ADPR-) |
(Sun et al., 2012) |
RP5481 |
| PAO1F exoT(ADPR-) |
(Sun et al., 2012) |
RP6202 |
| PAO1F exoS(GAP-) exoT(GAP-) |
(Sun et al., 2012) |
RP6203 |
| PAO1F exoS(ADPR-) exoT(ADPR-) |
(Sun et al., 2012) |
RP6205 |
| pP25-GFPo constitutive GFP producing plasmid, CarbR |
(Goodman et al., 2004) |
N/A |
| BL21/pET28b-TAT-Ras |
This study |
RE9356 |
| BL21/ pET28b-TAT-Ras(R41K) |
This study |
RE9357 |
|
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| Luminol |
Sigma-Aldrich |
123072 |
| Superoxide dismutase from human erythrocytes |
Sigma-Aldrich |
S9636 |
| Catalase from human erythrocytes |
Millipore |
219008 |
| Ficoll-Paque Plus (density 1.077 g/ml) |
GE Healthcare |
17-1440-03 |
| Dextran from Leuconostoc spp. (MR 450,00-650,000) |
Sigma-Aldrich |
31392 |
| 1x RBC Lysis Buffer |
eBioscience |
00-4333-57 |
| 100x Protease Inhibitor Cocktail |
Cell Signaling Technology |
5871 |
| 10x Cell Lysis Buffer |
Cell Signaling Technology |
9803 |
| DAPI Solution 1mg/mL |
Pierce |
62248 |
| Human PI3K (p120γ), Active full length recombinant protein expressed in Sf9 cells |
SignalChem |
P29-10H |
| NAD |
Sigma-Aldrich |
N1636 |
| Human 14-3-3 Zeta, histidine tagged |
Sigma-Aldrich |
Z3402 |
| Sulfo-NHS-LC-Biotin |
ThermoFisher |
21327 |
| PI3K inihibitor (AS-605240) |
Selleckchem |
S1410 |
| PI3K inhibitor (GDC-0941) |
Selleckchem |
S1065 |
|
|
|
| Critical Commercial Assays |
| Active Ras Detection Kit |
Cell Signaling Technology |
8821 |
|
|
|
| Experimental Models: Cell Lines |
| Human Peripheral Blood Neutrophils |
Case Western Reserve University: Hematopoietic Biorepository Core |
|
| C57BL/6J mice |
Jackson Laboratory |
000664 |
| B6.129S-Cybbtm1Din/J (gp91phox−/−) mice |
Jackson Laboratory |
002365 |
|
|
|
| Software and Algorithms |
| Metamorph Microscopy Automation and Image Analysis Software |
Molecular Devices |
|
| Prizm |
GraphPad Software |
|
