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. 2005 Jan 21;102(5):1324–1328. doi: 10.1073/pnas.0409409102

Fig. 3.

Fig. 3.

Hepcidin treatment markedly decreases FPN1 protein levels but does not affect light-chain ferritin (LFt) levels after erythrophagocytosis (EP). FPN1.RV2 J774 cells were incubated with or without EIgG for 1.5 h. After removal of noningested EIgG, macrophages were treated with or without 700 nM hepcidin and incubated for 3-6 h (Left) or 6-22 h (Right). Cell lysates (60 μg and 30 μg per lane) were blotted with anti-FPN1 antibody and anti-ferritin antiserum, respectively.

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