Table 1.

The effect of inhibitors, reducing agents, and divalent cations on the proteolytic activity of mirolysin.

Inhibitors and metal ions	Concentration	% of control
EDTA	1 mM	0
	10 mM	0
Pefabloc	10 μM	91
	100 μM	82
1,10-Phenanthroline	1 mM	2
	10 mM	2
Iodoacetic acid	5 mM	130
	10 mM	147
E-64	5 μM	115
	10 μM	115
ZnCl2	0.01 mM	112
	0.1 mM	96
CaCl2	1 mM	24
	10 mM	0
	0.1 mM	116
	1 mM	218
	5 mM	230
TLCK	10 mM	247
	5 mM	91
	10 mM	100
Leupeptin L-cysteine	50 μM	112
	1 mM	109
	10 mM	91
DTT	1 mM	98
	10 mM	74

Mirolysin activity determined at 37°C in 50 mM Tris-HCl, pH 8.0, using Azocoll as a substrate, was set at 100%.