Expression of VEGFR-2 during experimental glioma development. (a) During the second day after implantation, the tumor becomes vascularized from the base by CAM vessels positive for the transcript of VEGFR-2. (b) ByD4, the tumor is almost completely invaded by VEGFR-2-positive capillaries. (c) At D7, overall VEGFR-2 reactivity decreases. (d–f). Higher magnification shows endothelial cells positive for VEGFR-2 (arrows). (Scale bars: a–c, 0.5 mm; and d–f, 0.1 mm.)