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IL-2 secretion by murine 1.2 hybridomas when stimulated by the indicated glycolipids loaded on CD1d-coated plates. (A) IL-2 secretion profile of 3″-O-sulfo-α-GalCer compared with α-GalCer and analogs. (B) Dose-dependent secretion of IL-2 by Sphingomonas GSLs and analogs. CD1d molecules (10 μg/ml in PBS) were coated in a 96-well plate by incubation for 1 h at 37°C. IL-2 release was measured after 16 h of culture in a sandwich ELISA.
