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. 2017 Jun 20;7:3906. doi: 10.1038/s41598-017-04422-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ovariectomy increased OTM and bone resorption during OTM in OVX rats. (A,B) Representative images of tooth movement on day 21 of OTM in Sham and OVX rats. M1, the left maxillary first molar; M2, the left maxillary second molar; M3 the left maxillary third molar; the distance of OTM is indicated by a black line. (C) Effect of ovariectomy on the rate of OTM. Data represent means ± S.D. *P < 0.05, n = 6. (D–G) Representative images of TRAP staining in the pressure area on day 7 of OTM in Sham and OVX rats. E and G are high-magnification images of the squares in D and F respectively. AB, alveolar bone; DP, disto-palatal root; PDL, periodontal ligament; P, pressure area; T, tension area. (H) TRAP-positive osteoclasts in the pressure area during OTM in Sham and OVX rats. Data represent means ± S.D. *P < 0.05, n = 6.