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. 2017 Jun 20;7:3906. doi: 10.1038/s41598-017-04422-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ovariectomy promoted the expression of Runx2 and osteocalcin in the tension area produced by OTM in OVX rats. (A–D) Immunohistochemical staining of Runx2 or osteocalcin (Ocn) in the tension area on day 7 of OTM in Sham and OVX rats. (E,F) Number of Runx2- or osteocalcin-positive cells in the tension area during OTM. Data represent means ± S.D. *P < 0.05, n = 6.