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. 2017 Jun 20;7:3881. doi: 10.1038/s41598-017-03984-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Toolkit plasmids for efficient integration of DiCre recombinase into p230p or pfs47 loci of the P. falciparum genome. The CRISPR-Cas9 plasmid used here, pDC2-Cas9-hDHFRyFCU (derived from pDC2-Cas9-U6-hdhfr ²⁷) carries the SpCas9 nuclease gene and a single guide RNA cassette driven by a short U6 promoter and the hdhfr-yfcu fusion gene for selection. The rescue plasmids (pBSp230pDiCre and pBSPfs47DiCre) contain the genes for the split Cre-recombinase fused to FRB and FKBP12 respectively, flanked by regions of homology from the p230p or the pfs47 locus of P. falciparum. More detailed vector maps are available in Supplementary Fig. S2.