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Expression of neprilysin gene nep-1 by RT-qPCR in dry cysts, hydrated eggs, and hydrated eggs exposed to potato root diffusate (PRD) for 15 min, 1 h, 8 h, 24 h, 48 h and 7 days and hatched J2. Hydrated cyst expression level was use as calibrator. Error bars represent standard error of the mean and significant differences among treatments are indicated by different letters (Tukey).
