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. 2017 Jun 20;7:3879. doi: 10.1038/s41598-017-04223-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TIP60-deficient HONE6 cells show increasing frequency of stalled replication forks. (A) Labeling protocols for DNA fiber analysis. The HONE6 cells were treated with 10 μM cisplatin for 3 hours, followed by pulse-labelled with CldU and IdU for 20 minutes each. CldU was detected using a specific primary antibody and an Alexafluor594-labeled secondary antibody (red). IdU was detected using a specific antibody and an Alexafluor488-labeled secondary antibody (green). Representative images of replication tracks of HONE6 were shown as indicated (B). (C) Quantification of stalled forks was determined from at least 100 DNA fibers.