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. 2017 Jun 20;7:3896. doi: 10.1038/s41598-017-04248-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methylation of gene specific CpGs in lung cancer cell lines. (A) CpG sites are annotated depending on the gene specific orientation in TSS1500 (−1500 to −200 bp), TSS200 (−200 bp to TSS), 5′-UTR, 1^st exon, gene body and 3′-UTR. (B) Methylation levels of CpG islands and flanking N-and S-shores were analyzed in NHBEC and in the lung cancer cell line H322 by 450 K bisulfite bead chip arrays according to the gene specific annotation.