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. 2017 Jun 20;7:3896. doi: 10.1038/s41598-017-04248-w

Figure 3.

Figure 3

Increased 5′-shore methylation in promoter and coding regions. (A) Depending on the chromosome orientation genes are transcribed from the p- to the q arm (Watson strand genes) or in the opposite direction (Crick strand genes). For Watson strand genes the CGI flanking N-shores are the 5′-shores and for Crick strand genes S-shores are the 5′-shores. For further details are provided in Fig. 2. (B) Methylation levels of N- and S-shore gene specific regions in 69 NSCLC cell lines (ABC-1, SW1573, HOP18, HCC1171, LXFL529, H1703, H441, EBC-1, H322, A427, H1568, CHA-GO-K-1, H2087, H1975, H226, H2444, HCC515, H1299, H2030, H2405, HCC2935, H1373, H1666, RERF-LC-Ad1, Calu-6, H1155, H1651, H2347, H358, H838, H2228, HCC827, H2073, H1650, H23, H1993, Calu-3, H650, H460, H727, A549, H292, HCC4006, H2170, H1838, H820, H1355, RERF-LC-MS, H2122, H1793, H661, HOP62, HCC15, EKVX, H1792, H2110.1, Calu-1, H2110, HCC4017, H2009, HOP92, SK-MES-1, RERF-LC-KJ, H1437, H647, H2126, H2172, H1435 and H1755), six normal lung cell lines (NLC): gBEC1, gBEC1_UI, gSAC1, gSAC1_UI, gBEC and gSAC) and five normal lung tissues (NLT): GSM1264690, GSM1264711, GSM1264727, GSM1264764, GSM1264854). Methylation data were obtained from NLCBI-GEO-Accession: GSE36216 and GSE5240121, 22. Promoter regions are defined as the TSS1500 (−1500 to −200), TSS200 (−200 to TSS), 5′-UTR, 1st exon and coding regions consisted of the gene body and 3′-UTR.