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. 2005 Jan 21;102(5):1318–1323. doi: 10.1073/pnas.0409017102

Fig. 2.

Fig. 2.

Western blotting of ApGSMT and ApDMT and the accumulation of betaine in Synechococcus sp. PCC 7942 cells expressing ApGSMT/ApDMT. (A) Synechococcus sp. PCC 7942 cells were grown in BG11 medium containing the indicated concentrations of NaCl. The soluble fractions of Synechococcus cells expressing ApGSMT/ApDMT were prepared by sonication. Equal amounts of protein (50 μg) were subjected to 12.5% SDS/PAGE and visualized by using antibodies raised against ApGSMT and ApDMT. (B) Betaine content in wild-type, ApGSMT/ApDMT, and bet-cluster gene-expressing cells. Wild-type, ApGSMT/ApDMT, and bet-cluster gene-expressing cells were harvested at the logarithmic phase. Betaine was extracted as described in Materials and Methods. Betaine content was analyzed by time-of-flight mass spectroscopy. Data are the means ± SD of three independent experiments, with three replicates per experiment.