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. 2017 Jun 20;7:3860. doi: 10.1038/s41598-017-04170-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AAV-shRNA treatment decreases HBsAg and HBcAg in hepatocytes of HBV-persistent mice. Serum and liver samples of mice treated with AAV2-shRNA were collected at the indicated time points during a 6 month period. Liver sections were fixed and stained for HBsAg (a) and HBcAg (c). Ten random fields were selected per slide, and the percentages of HBsAg- (b) and HBcAg-positive (d) hepatocytes were quantified using Image-Pro Plus. P < 0.05: significant difference, Data represent the mean ± SD.