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. 2017 Jun 20;7:3914. doi: 10.1038/s41598-017-04305-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of P301L hTau in MEC induces endogenous hippocampal tau hyperphosphorylation and accumulation. (A) Phosphorylation level of tau at Ser396 was significantly increased in MEC and its projection fibers to DG in brain slices one month after transduction of P301L hTau in MEC, where phosphorylated tau were co-localized with GFP. Interestingly, transduction of P301L hTau in MEC induced an increase of phosphorylated tau in the granular layer (gl) shown in magnified box, suggesting endogenous hippocampal tau hyperphosphorylation. (B) Western blotting showed that the phosphorylation of 55 kD tau at Ser396 or Ser199 was significantly increased in hippocampus and cortex, while only around 80 kD band (tau+GFP) was found in cortex of P301L mice. An increase of total tau probed by tau-5 suggested tau accumulation (n = 3 per group, *p < 0.05, **p < 0.01). Blots of Ser396 and Ser199 were overexposed here for the expressions of exogenous GFP labeled tau was much less than internal tau. Cropped blots are displayed here and full-length blots are included in the supplementary information. Data were expressed as mean ± SEM.