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. 2017 Jun 20;7:3914. doi: 10.1038/s41598-017-04305-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of P301L hTau in MEC suppresses the neuronal activity without causing significant cell death. (A–F) Nissl staining indicated that the numbers of neurons were not changed in P301L hTau transduced mice in both MEC and DG regions compared to control (scale bar in A and B, 500 μm; in C-F, 100 μm). (G) Quantitative analysis of the Nissl staining showed that P301L hTau transduction didn’t lead to neuronal death (n = 3 per group). (H–M) c-fos was employed to detect the neuronal activity. A density of the c-fos puncta in both MEC and DG subsets was remarkably decreased in P301L hTau-transduced mice compared with the control. (scale bar in H and I, 500 μm; in J-M, 100 μm). (N) Quantification of the number of c-fos positive cells per 1 mm² revealed that P301L hTau lowered the c-fos expression in the MEC and DG. (n = 3 per group, 9 slices, *p < 0.05, **p < 0.01). (O–S) Whole-cell patch results suggested that AP amplitude was significantly lowered in P301L hTau cells compared to control (O,P). However, passive properties were not altered in P301L hTau cells since their membrane resting potential (Q) was unchanged compared to controls. There was no difference in threshold (R) or half width (duration, (S)) between control and P301L hTau cells (n = 3 per group, 9 slices, *p < 0.05). Arrows in J-M were used to indicate the cFos staining. Data were expressed as mean ± SEM.