Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 20;7:3868. doi: 10.1038/s41598-017-04232-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Increased level of GTP-bound Rab5 and Rab7 was detected in Line 78 E18 brain samples. Brain lysates were incubated with GTP-agarose beads to pull down activated GTP-bound Rab5 (A) as described in Materials and Methods. The levels of GTP-Rab5 in these samples were quantitated and normalized against internal control. Samples from Line 78 mice show an 89% increase over non transgenic controls (B). Similarly, activated Rab7 was pulled down (C) and a significant increase of GTP-Rab7 is seen in PDGF-β-ASYN-GFP mice as compared to non-transgenic controls (D). For B and D, results are from 6 animals per group. ***p < 0.01. Mouse brain homogenates were immunoprecipitated with an antibody recognizing dynein intermediate chain (DIC). Immunoprecipitates were analyzed by SDS-PAGE/immunoblotting with antibodies as indicated (E). At least three independent experiments were performed for all the studies presented. When possible, all original, full size, uncut blots are presented in Supplemental Information. Otherwise, the original blots are presented in Supplemental Information.