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. 2017 Jun 20;7:3868. doi: 10.1038/s41598-017-04232-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of GFP-ASYN induced enlargement of Rab5 early endosomes in PC12 cells. PC12 cells were transfected with either GFP + mCherry-Rab5^WT (A) or GFP-ASYN + mCherry-Rab5^WT (B) for 48 hrs. Enlarged mCherry-Rab5 early endosomes typically seen in GFP-ASYN cells were indicated with arrow heads (B). The size of mCherry-Rab5 in A and B was quantitated and presented in C; and the size distribution of the mCherry-Rab5 early endosomes was presented in D. The p values are indicated and n.s. = non-significant.