Table 1.
Capability of fish Fads2 for Δ6 desaturation of C24 substrates 24:4n−6 and 24:5n−3 using a yeast Saccharomyces cerevisiae heterologous system as described in Materials and Methods. Fatty acid (FA) conversions were calculated as the percentage of 24:4n−6 and 24:5n−3 desaturated to 24:5n−6 and 24:6n−3, respectively, as [product area/(product area + substrate area)] × 100. Conversions towards the control FA substrate (18:3n−3 as controls for Δ6 and Δ6Δ5 desaturases, 20:4n−3 for Δ5 desaturases and 22:5n−3 for Δ4 desaturases) are also indicated. In order to normalise the % conversions obtained throughout the Fads2 dataset, ratios between the activities on 24:5n−3 and those on the control FA (“Δ24:5n−3/Δcontrol”) are also presented.
| Desaturasea | % Conversion | |||
|---|---|---|---|---|
| 24:4n−6 → 24:5n−6 | 24:5n−3 → 24:6n−3 | Control → Product | Δ24:5n−3/Δcontrol | |
| ScyΔ6Fads2 | 29.3 | 34.3 | 41.9 | 0.82 |
| AgΔ6Fads2 | 25.4 | 19.0 | 15.3 | 1.24 |
| AjΔ6Fads2 | 14.0 | 15.8 | 17.8 | 0.89 |
| DrΔ6Δ5Fads2 | 10.4 | 15.8 | 11.9 | 1.33 |
| CgΔ6Δ5Fads2 | 29.9 | 28.1 | 31.5 | 0.89 |
| SsΔ6Fads2 | 18.5 | 26.0 | 23.9 | 1.09 |
| SsΔ5Fads2 | 1.4 | 6.4 | 3.4 | 1.88 |
| OmΔ6Fads2 | 7.5 | 19.7 | 20.4 | 0.97 |
| CeΔ6Δ5Fads2 | 4.2 | 9.0 | 22.9 | 0.39 |
| CeΔ4Fads2 | ND | ND | 9.9 | 0.00 |
| ScΔ6Δ5Fads2 | 6.0 | 7.4 | 36.4 | 0.20 |
| ScΔ4Fads2 | ND | ND | 6.9 | 0.00 |
| SaΔ6Fads2 | 4.8 | 6.5 | 15.0 | 0.43 |
| NmΔ6Fads2 | ND | ND | 10.5 | 0.00 |
| OnΔ4Fads2 | ND | ND | 4.5 | 0.00 |
ND, Not detected
aScy, Scyliorhinus canicula; Ag, Arapaima gigas; Aj, Anguilla japonica; Dr, Danio rerio; Cg, Clarias gariepinus; Ss, Salmo salar; Om, Oncorhychus mykiss; Ce, Chirostoma estor; Sc, Siganus canaliculatus; Sa, Sparus aurata; Nm, Nibea mitsukurii; On, Oreochromis niloticus.