Figure 2.

(A) ¹³C-Excess (mol%) and (B) the fractional isotopolog distributions (%) in key metabolites of Francisella tularensis subspecies holartica WT strain (beaver isolate) (Fth Beaver, 1), the Francisella tularensis subspecies holartica life vaccine strain LVS (Fth LVS, 2), or the less pathogenic Francisella novicida strain U112 (Fno, 3) grown in medium T supplied with 11 mM [U-¹³C₆]glucose. ¹³C-Excess (mol%) and relative fractions of isotopologs (%) were determined by GC/MS of silylated derivatives at the indexed m/z values. Error bars indicate standard deviations from the means of 6 to 9 values 2–3 × biological replicates, 3 × technical replicates. M+1, M+2, M+3, etc. indicate isotopologs carrying 1, 2, 3, etc. ¹³C-atoms. For numerical values, see Supplemental Tables S1, S5, S9, S13, S17, S21.