Figure 4.

PAK4 promotes the activation of NF-κB and AP-1 in JEV-infected U251 cells. U251 cells were transfected with siPAK4 or siNC followed by JEV infection and were harvested at 12 h post infection. The nuclear translocation of p65, protein level of IκBα (A) and expression and phosphorylation of JUN (B) were detected by Western-blot. Levels of nuclear localized p65, IκBα and phosphorylated JUN were quantified with immunoblot scanning and normalized to the amount of GAPDH and Lamin A. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05, compared with cells transfected with siNC (n = 3).