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. 2017 Jun 21;7:264. doi: 10.3389/fcimb.2017.00264

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Copyright © 2017 Ibrahim, Peter, Wagener, Drillich, Ehling-Schulz, Einspanier and Gabler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Normalized mRNA expression of the virulence factors: (A) PLO, (B) cbpA, (C) nanH, (D) nanP, (E) fimA, (F) fimC, (G) fimE, and (H) fimG in TP2 and TP5 cultured in BHI supplemented with 5% HI FCS up to 24 h. The bars show means ± SEM of mRNA expression values from different inocula (n = 3). Asterisks above the bars indicate significantly different mRNA expression (P < 0.05) between the two strains at the same time point. Different capital letters (A,B) above the bars indicate a significant difference within TP2 between different time points (P < 0.05).