Table 1.

Selected known and putative T. pyogenes virulence gene transcripts, primer sequences, and annealing temperatures used for PCR/qPCR with expected amplicon size.

Gene symbol	Accession no./references	Primer sequence (5′ → 3′)	Annealing temperature	Amplicon size (bp)
plo	AB027461.1	F: AAG TAT CCT GAC CAT GCT GC	60°C	228
		R: GCC GAA AAC GCT ATG TGG AG
cbpA	AY223543.1	F: TAC TGT TCG TCC AAC TCG CAT C	60°C	110
		R: TGC CCG GCT TGA TAT AAC CTT C
nanH	AF298154.1	F: CAC GGA CGT GAA GAG CTT TG	63°C	243
		R: ACT TCA ACC TTC GGC TCT GG
nanP	AY045771.1	F: GAT GAC GCA AAC AAG ACG CC	60°C	152
		R: GTC AGC ACA AAA CCA GCC AG
fimA	Silva et al., 2008	F: CAC TAC GCT CAC CAT TCA CAA G	57°C	605
		R: GCT GTA ATC CGC TTT GTC TGT G
fimA-Q	this study	F: CCG TTC CTC GTT ACC CTT CC	60°C	152
		R: CAG GTA ATC TCA GCA CCG GG
fimC	Silva et al., 2008	F: TGT CGA AGG TGA CGT TCT TCG	60°C	843
		R: CAA GGT CAC CGA GAC TGC TGG
fimC-Q	this study	F: GCC GTT CGC TTC ACA CTT AC	60°C	137
		R: ATG GCA AAA CCA AAG ACG CC
fimE	Silva et al., 2008	F: GCC CAG GAC CGA GAG CGA GGG C	55°C	775
		R: GCC TTC ACA AAT AAC AGC AAC C
fimE-Q	this study	F: CGC CCG TTC TTC TTT GCT TC	60°C	143
		R: TGC CTC GTT GAG ACC AAG TC
fimG	Silva et al., 2008	F: ACG CTT CAG AAG GTC ACC AGG	57°C	929
		R: ATC TTG ATC TGC CCC CAT GCG
fimG-Q	this study	F: GTA GCC GGA GTT GAG GAA GG	60°C	157
		R: ATC CTC GCT CTC TTG CTG TG
16S rRNA	NR_044858.1	F: AAG ACC GGG GCT TAA CTT CG	60°C	133
		R: AGT AAC CTG CCT TCG CCA TC
smc	U84782.2	F: ATG ATC ACA CTC CCG CAA CC	60°C	119
		R: GGG TTG ATC TTG CCC AAA CG