Table 2.

Selected bovine gene transcripts, primer sequences, and annealing temperatures used for quantitative PCR with expected amplicon size.

Gene symbol	Accession no./references	Primer sequence (5′ → 3′)	Annealing temperature	Amplicon size (bp)
18S rRNA	Odau et al., 2006	F: GAG AAA CGG CTA CCA CAT CCA A	61°C	337
		R: GAC ACT CAG CTA AGA GCA TCG A
ACTB	Gärtner et al., 2015	F: CGG TGC CCA TCT ATG AGG	58°C	266
		R: GAT GGT GAT GAC CTG CCC
GAPDH	Gärtner et al., 2015	F: CCC AGA AGA CTG TGG ATG G	62°C	306
		R: AGT CGC AGG AGA CAA CCT G
SDHA	Gärtner et al., 2015	F: GGG AGG ACT TCA AGG AGA GG	60°C	219
		R: CTC CTC AGT AGG AGC GGA TG
CXCL3	Gärtner et al., 2016	F: GCC ATT GCC TGC AAA CTT	56°C	189
		R: TGC TGC CCT TGT TTA GCA
IL8	Fischer et al., 2010	F: CGA TGC CAA TGC ATA AAA AC	56°C	153
		R: CTT TTC CTT GGG GTT TAG GC
IL1A	Gabler et al., 2009	F: TCA TCC ACC AGG AAT GCA TC	59°C	300
		R: AGC CAT GCT TTT CCC AGA AG
IL6	Konnai et al., 2003	F: TCC AGA ACG AGT ATG AGG	56°C	236
		R: CAT CCG AAT AGC TCT CAG
PTGS2	Odau et al., 2006	F: CTC TTC CTC CTG TGC CTG AT	60°C	359
		R: CTG AGT ATC TTT GAC TGT GGG AG