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. 2017 Jun 21;7:259. doi: 10.3389/fcimb.2017.00259

Figure 2.

Figure 2

Immunofluorescence staining of Ly6G+ neutrophil granulocytes and T. gondii in brain slices. Immunostaining of PECAM-1 or SAG1 or CST1 (green), Ly6G (red), and Sytox Dead Cell stain (blue) in the cortex of T. gondii-infected C57BL/6 mice (A–C). (A) In acute cerebral toxoplasmosis, recruitment of Ly6G+ neutrophil granulocytes into the brain from the circulation is shown. (B) Further, massive infiltration of Ly6G+ neutrophil granulocytes around T. gondii tachyzoites (green) is shown. (C) In chronic cerebral toxoplasmosis, no localization of Ly6G+ neutrophil granulocytes is shown. Eight coronal slides per mouse were analyzed with a maginification of 63x; n = 4 mice. This experiment was repeated two times. Scale bars, 20 μm in (A–C).