Figure 6.

Selective depletion of Ly6G⁺ neutrophil granulocytes. C57BL/6 mice were infected with T. gondii. From day 10 to day 23 post-infection mice were alternatively treated with either IgG mAb (control, left), or anti-Ly6G (Anti-Ly6G, right) to deplete neutrophil granulocytes. The cells were gated as shown in Figures 1E–H. Panel (A) displays representative plots to define CD11b⁺Ly6G⁺ circulating neutrophil granulocytes (upper gate) in the blood. (B) The bar graph represents the percentage of Ly6G⁺ cells in the blood. (C) The upper plots show the gating of lymphocytes (CD11b⁻CD45^hi), activated microglia (CD11b⁺CD45^int) and the myeloid population (CD11b⁺CD45^hi) in infected brains. Middle plots display the myeloid subsets: Ly6G⁺(neutrophil granulocytes) and Ly6G⁻ (monocytes) in the brain. Lower plots display the inflammatory monocyte subset (from the Ly6G⁻ gate): Ly6C^hi. (D) The bar graphs represent the total cell number of the respective subsets in the brain (black bars: control; white bars: anti-Ly6G). (E) The bar graphs represent the total cell number of the respective CD4⁺ and CD8⁺ lymphoid cell population. Data shown here is the representative of 2 individual experiments with 4 mice per group for each experiment. The numbers in the representative contour plots are % of the parent population. Significant differences (^*p < 0.05, ^**p < 0.01, ^***p < 0.001) were determined using the Mann-Whitney test.