Figure 7.

Semi-quantitative RT-PCR analysis of Ly6G⁺ neutrophil granulocytes-depleted mice. Quantitative and Semi-quantitative RT-PCR analyses of pathogen load and cytokine gene expression in brains of T. gondii-infected C57BL/6 mice (for 23 days) after treatment with IgG mAb (black bars) or anti-Ly6G (white bars). (A–E) Relative expression was calculated by normalization to the expression of the housekeeping genes mouse argininosuccinate lyase and hypoxanthine phosphoribosyltransferase. Resulting data were further normalized on mean values of control groups. Data shown is the representative of 2 individual experiments with 4 mice per group for each experiment. Significant differences (^*p < 0.05, ^**p < 0.01, ^***p < 0.001) were determined using the Mann-Whitney test.